Western blot to detect specific proteins in different cell types and/or tissues.
Northern blot to detect specific RNA sequences in cell types and/or tissues, and also to examine RNA integrity.
Design oligonucleotides (e.g. primer/probe) for PCR and DNA sequencing, as well as using different computer programs to analyze the results.
qPCR and RT-qPCR to examine gene or microRNA expression.
Run toxicity assay to assess drug effects on in vivo models.
Develop and run different types of assays and ELISA, including colorimetric, florescence, luminescence, etc.
Developed a protocol for assessing proliferation and apoptosis over time in different cell lines, with time points going out to 2 weeks post treatment.
Use of Flow to examine different cell populations and their phenotypic changes over time with treatment.
Genetic Engineering and DNA Manipulation
Insertion and deletions of gene sequences using restriction enzymes and ligase or Gibson assembly.
Amplify DNA segment of interest using PCR techniques and/or cloning it into bacteria.
In depth understanding of CRISPR-Cas9 systems, analyzing indels using T7E1 and TIDE, as well as using sequencing to examine insertions and deletions.
Programs and Instruments
Microscopy: Light, Florescent, Dissecting Microscopes, Confocal, etc.
Use of multiple laboratory equipment: nanodrop, plate readers, Viia7, Cytoflex, BioRad chemidoc, thermocycler, liquid handlers, Nexcelom, Nova, Countess, etc.
Programs: MS Word, Excel, PowerPoint, Outlook, GraphPad, FlowJo, ImageJ, electronic notebooks, Google Docs, MatLab, etc.
